| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Qiu, Selina Y (12)
|
GUSHAC |
33.83 |
32.67 |
-1.16 |
| 2 |
Boettcher, Kadyn S (12)
|
GUSWAT |
33.99 |
33.24 |
-0.75 |
| 3 |
Woolery, Livia E (12)
|
GUAQUA |
33.95 |
33.73 |
-0.22 |
| 4 |
Odom, Kaylee C (11)
|
GUAQUA |
33.68 |
33.93 |
0.25 |
| 5 |
Cardenas, Isabella K (11)
|
GUSHAC |
37.65 |
36.20 |
-1.45 |
| 6 |
Painter, Reese E (12)
|
GUSHAC |
39.91 |
36.74 |
-3.17 |
| 7 |
Cowan, Ella L (12)
|
GUCOOG |
36.93 |
36.87 |
-0.06 |
| 8 |
Miller, Molly M (12)
|
GUSWAT |
44.01 |
36.97 |
-7.04 |
| 9 |
Asthagiri, Maithreyi D (11)
|
GUCOOG |
|
40.46 |
- |
| 10 |
MacLean, Rowan H (12)
|
GUAQUA |
42.75 |
41.51 |
-1.24 |
| 11 |
Orlina, Marisol E (11)
|
GUCOOG |
42.95 |
41.99 |
-0.96 |
| 12 |
Andrade, Mia A (11)
|
GUSSS |
46.49 |
42.53 |
-3.96 |
| 13 |
Sliger, Madalynn E (11)
|
GUTCAP |
41.05 |
43.29 |
2.24 |
| 14 |
Fraley, Katelyn E (12)
|
GUSWAT |
53.42 |
43.62 |
-9.80 |
| 15 |
Aguilera, Idaly E (11)
|
GUHCAP |
43.51 |
43.78 |
0.27 |
| 16 |
Delasbour, Tatyana J (11)
|
GUHCAP |
|
45.30 |
- |
| 17 |
Clements, Ariel Z (11)
|
GUSSS |
45.23 |
45.84 |
0.61 |
| 18 |
Brown, Kennedy J (11)
|
GUAQUA |
52.41 |
46.26 |
-6.15 |
| 19 |
Gow, Alexis K (11)
|
GUSWAT |
53.93 |
47.38 |
-6.55 |
| 20 |
Massey, Jubilee G (11)
|
GUHCAP |
|
47.59 |
- |
| 21 |
Blanco, Sofia M (11)
|
GUSHAC |
|
48.27 |
- |
| 22 |
Wilburn, Savannah T (12)
|
GUTCAP |
1:10.11 |
49.09 |
-21.02 |
| 23 |
Garcia, Leslie F (11)
|
GUAQUA |
55.60 |
51.69 |
-3.91 |
| 24 |
Moultrie, Alden S (11)
|
GUTTST |
|
1:01.41 |
- |
|
Ayala, Valeria A (11)
|
GUHCAP |
|
NS |
- |